| Gene name: | PLD2 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | IMR-90 |
| Experiment: | SA-β-gal activity assay//Knockdown |
| Description: | We downregulated PLD2 using siRNA in proliferating IMR-90 cells (PDL 32) and found an increased rate of SA-β-gal staining in proliferating cells, compared with the control cells. |
| Target gene: | CK2α |
| Official symbol(s): | CSNK2A1 |
| R-AG-Target gene: | Inhibition |
| Subcategory: | Unclear |
| Target gene experiment: | SA-β-gal activity assay//Knockdown |
| Target gene description: | Proliferating IMR-90 (PDL 32) and HCT116 cells were transfected with PLD2 siRNA, and a significant increase in the SA-β-gal activity was found in response to PLD2 knockdown, whereas co-transfection of cells with CK2α resulted in a significant decrease in the level of SA-β-gal staining. |
| Regulatory pathway: | P53-P21 |
| R-AG-Pathway: | -- |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | Knockdown//SA-β-gal activity assay//Western blot |
| Pathway description: | In the wild-type HCT116 cells, significant increase in SA-β-gal activity in response to PLD2 knockdown was observed. However, p53- and p21Cip1/WAF1-negative cells did not show senescence；When the protein levels of p53 and p21Cip1/WAF1were determined by Western blot, the expression of p53 was found to be upregu-lated in both the wild type and p21Cip1/WAF1-/- cells treated with PLD2 siRNA, but not in p53-/-cells. The expression of p21Cip1/WAF1 was upregulated only in the wild-type HCT116 cells treated with PLD2 siRNA. |
Annotation:
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