| Gene name: | PRKCA |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HEK293,HCT116 |
| Experiment: | SA-β-gal activity assay |
| Description: | Inhibition of cPKC or aPKC resulted in increased SA-β-gal staining as compared with control. |
| Regulatory pathway: | AKT-FOXO3A-ROS-P53-P21 |
| R-AG-Pathway: | -- |
| Official symbol(s): | AKT1-FOXO3-ROS1-TP53-CDKN1A |
| Pathway experiment: | Immunostaining//Flow cytometry//Western blot |
| Pathway description: | Treatment with triciribine markedly abolished PKC inhibition-mediated nuclear exportation of FoxO3a；We observed an increase in ROS accumulation, as shown by the rightward shift in DCF and ETH fluorescence in cells due to PKC downregulation；Accumulation of p53 and p21Cip1/WAF1 proteins was also observed in cells treated with G?o6983 or Ad-DN-PKCz, as compared with untreated control cells, suggesting that downregulation of cPKC and aPKC induces senescence through stabilization of p53 and transcriptional activation of p21Cip1/WAF1. |
Annotation:
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