| Gene name: | NEDD4 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | IMR-90,2BS |
| Experiment: | SA-β-gal activity assay |
| Description: | Nearly all of the NEDD4-1-shRNA-infected cells displayed stronger levels of blue SA-β-gal staining similar to senescent cells, the control cells showed a lower frequency of SA-β-gal staining. However, only sporadic SA-β-gal-positive cells were seen in NEDD4-1 -transfected cells, although the corresponding vector control cells showed strong SA-β-gal staining. |
| Target gene: | PPARγ |
| Official symbol(s): | PPARG |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | A high level of NEDD4-1 overexpression also caused a decrease of endogenous PPARγ protein in the Hela cells. Such a decrease of PPARγ protein level can be blocked by proteasomal inhibitor MG132；we demonstrated that endogenous NEDD4-1 negatively regulates PPARγ levels quantitatively following increased RNAi concentration. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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