| Gene name: | HELLS |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | 2BS,WI-38 |
| Experiment: | SA-β-gal activity assay//Cell growth assay//Flow cytometry//Knockdown |
| Description: | Nearly all of the Lsh–shRNA-infected cells (PD 42) showed strong levels of blue SA-β-gal staining similar to senescent cells;Lsh blocks the formation of senescence-associated heterochromatin foci (SAHF); growth of Lsh-HA-transfected cells advanced quickly, suggesting a strong proliferative potential relative to the control cells . In contrast to the severe G1 cell-cycle arrest imposed by Lsh-shRNA, the control-shRNA cells showed a decrease in the proportion of 2BS cells in the G0/G1 phases. |
| Target gene: | P16//HDAC |
| Official symbol(s): | P16//HDAC9 |
| R-AG-Target gene: | Inhibition//-- |
| Subcategory: | Acetylation |
| Target gene experiment: | Western blot//RT-PCR//Pull-down assay//IP |
| Target gene description: | A marked increase in p16?INK4a?protein levels in Lsh-shRNA cells comparing to control-shRNA cells.Lsh-shRNA-infected cells showed a significant increase in p16 mRNA levels in 2BS cells .The results showed that Lsh immunoprecipitates from Lsh-deficient cells contained significantly decreased the reverse immunoprecipi- tation displayed the similar result. In addition, GST pull-down experiments with bacteria expressed GST-Lsh and in vitro transcripted/translated HDAC1 were then performed to examine the nature of the interaction between Lsh and HDAC1. HDAC1 levels relative the mock-transfected cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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