| Gene name: | ERCC6 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | IMR-90 |
| Experiment: | Knockdown//SA-β-gal activity assay |
| Description: | Proliferation arrest of CSB-knocked down fibroblasts earlier (PN26， PN24) compared to cells transduced with shSCR (PN28). Precocious senescence upon transient CSB knockdown was confirmed by a higher number of SA-β-gal+ cells. |
| Target gene: | P21 |
| Official symbol(s): | P21 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Binding to promoter |
| Target gene experiment: | CHIP//qRT-PCR |
| Target gene description: | CSB directly interacts with two regions of the?p21Waf1?promoter that are target of p5329, and a more extended interaction in the distal (5’) region was observed with the C-terminal anti-CSB than with N-terminal anti-CSB. |
| Regulatory pathway: | DDR-P21 |
| R-AG-Pathway: | -- |
| Official symbol(s): | CDKN1A |
| Pathway experiment: | Immunostaining//Western blot//SA-β-gal activity assay |
| Pathway description: | Decreased CSB levels upon irradiation resulted in the loss of CSB interaction with the p21Waf1 promoter ；Senescence following irradiation was verified by high levels of the DDR-dependent senescence marker p21. |
Annotation:
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