| Gene name: | NOL12 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | Skin |
| Cell name: | HDF |
| Experiment: | BrdU assay//SA-β-gal activity assay//Flow cytometry |
| Description: | Proliferation rate of NOL12-depleted cell cultures, confirmed by a decreased percentage of living cells that incorporated 5-ethynyl-2′-deoxyuridine (EdU), with cycling cells from NOL12-depleted cell cultures exhibiting a cell cycle delay in comparison to the cycling of the control；enescence-associated β-Gal positive . |
| Regulatory pathway: | P53 |
| R-AG-Pathway: | -- |
| Official symbol(s): | TP53 |
| Pathway experiment: | Immunostaining//Western blot//Knockdown |
| Pathway description: | Both immunoblot and immunofluorescence quantitative analyses of p53 levels revealed its stabilization upon NOL12 and XRN2 knockdowns?; RPL11 to be required for p53 activation upon NOL12 knockdown. upon NOL12 knockdown, we detected a significant induction of the p53 downstream target p21/CDKN1A. |
Annotation:
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