| Gene name: | MKRN1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | AGS,SNU601,MEF |
| Gene ID: | 23608 |
| Category: | protein coding |
| Phenotype: | Gastric cancer |
| Experimental category: | HL |
| PMID: | 23104211 |
| Experiment: | SA-β-gal activity assay//Knockdown |
| Description: | Upon ablation of MKRN1, both cells exhibited growth retardation and senescence acceleration detected using SA-β-galactosidase staining. The results indicated that 53% of MKRN1?/? MEFs that were counted displayed premature senescence, whereas only 7.3% of wild-type MEFs went through senescence at passage 6 (MKRN1+/+ vs MKRN1?/?, mean percentage = 7.3% vs 53%, difference = 45.7%, 95% CI = 39.07% to 52.33%, P =0.005). |
| Target gene: | P14 |
| Official symbol(s): | P14 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | MKRN1 induced degradation of exogenous p14ARF in H1299 cells in a concentration-dependent manner. Experiments employing cycloheximide (CHX) treatment to measure the protein half-life showed that approximately half of the exogenous p14ARF protein disappeared in less than 2h in the presence of MKRN1, compared with almost 5h without MKRN1. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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