| Gene name: | PRKN |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | Lung |
| Cell name: | Airway epithelial cell |
| Gene ID: | 5071 |
| Category: | protein coding |
| Phenotype: | Chronic obstructive pulmonary disease |
| Experimental category: | L |
| PMID: | 30290714 |
| Experiment: | Western blot//ELISA//SA-β-gal activity assay//Immunohistochemical staining |
| Description: | Senescence-associated-GLB1/β-galactosidase staining showed clear positive staining of airway epithelial cells in CS-exposed prkn KO mice but few positive staining cells were detected in CS-exposed wild-type mice.Immunohistochemical staining and western blotting of CDKN1A/p21 and CDKN2A/p16 (senescence-associated cyclin dependent kinase inhibitors) were performed. Both airway epithelial cells and alveolar epithelial cells in CS-exposed prkn KO mice demonstrated clear positive staining in both CDKN1A and CDKN2A . Consistent with the degree of cellular senescence, CXCL1 and IL1B protein levels were significantly increased in CS-exposed prkn KO mice compared to CS-exposed wild-type mice . |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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