| Gene name: | ARHGAP18 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HUVEC |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis |
| Description: | After 48 h, ARHGAP18 overexpression resulted in a significant increase in large cells, defined as senescent based on size and flattened appearance,expression of SA-β-galactosidase (SA-β-gal) and of p21 expression. |
| Target gene: | AP1 |
| Official symbol(s): | JUN |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | ARHGAP18-senescent cells also prevented the phosphorylation of c-Jun, an essential component of the transcription factor AP-1, which has also been shown to regulate components of the SASP and for adhesion molecule expression on EC. |
| Regulatory pathway: | NF-κB |
| R-AG-Pathway: | Upregulation |
| Official symbol(s): | NFKB1 |
| Pathway experiment: | Western blot |
| Pathway description: | In the absence of TNFa stimulation, both EV and ARHGAP18-senescent cells showed basal levels of NF-jB expression. Following TNFa stimulation, EV cells showed increased expression of the RelA/p65 subunit of the NF-kB complex , whereas the ARHGAP18-senescent cells did not show this same upregulation. |
Annotation:
Loading,please wait...