| Gene name: | FOXA1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HEC-1B |
| Gene ID: | 3169 |
| Category: | protein coding |
| Phenotype: | Endometrial cancer |
| Experimental category: | L |
| PMID: | 27349269 |
| Experiment: | SA-β-gal activity assay//MTT assay |
| Description: | Restoring FOXA1 expression induced morphological features of senescence, including accumulation of granular cytoplasmic inclusions and elevated β-galactosidase activity.MTT and plate colony formation assays revealed that HEC-1B cells transiently transfected with a FOXA1 expression plasmid showed significant growth inhibition and limited colony formation compared with the cells transfected with the negative control vector. |
| Target gene: | P16 |
| Official symbol(s): | P16 |
| R-AG-Target gene: | Activation |
| Subcategory: | Unclear |
| Target gene experiment: | qRT-PCR |
| Target gene description: | Following treatment with LY 294002, expression of the senescence-associated genes p16INK4a and p27kip1 was markedly increased, while PTEN gene expression was decreased. |
| Regulatory pathway: | AKT |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | AKT1 |
| Pathway experiment: | Western blot |
| Pathway description: | Western blot analysis of the protein levels of p(Ser473)-AKT and AKT revealed almost complete inhibition of p-AKT expression in cells treated with LY 294002 at 1, 10 and 20 μM, with optimal inhibition achieved at 10 μM. |
Annotation:
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