| Gene name: | DNMT1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HDF |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | Upregulation of DNMT1 was found to partially reverse the observed UVA-induced increase in SAβ-gal positive cells.DNMT1 expression attenuated the observed increase in p53, p21, and p16 expression following UVA irradiation. |
| Target gene: | ZEB1 |
| Official symbol(s): | ZEB1 |
| R-AG-Target gene: | Upregulation |
| Subcategory: | Binding to promoter Methylation |
| Target gene experiment: | Luciferase reporter assay//SA-β-gal activity assay//Western blot |
| Target gene description: | Compared with the control vector, ZEB1 over-expression caused a significant increase in luciferase activity in the cells that were cotransfected with the WT and Mut2-4 reporter vectors. Conversely, cotransfection of ZEB1-expressing vector with the Mut1 reporter vector gave a level of luciferase activity that was equivalent to the control.ZEB1 up-regulation partially reversed the UVA-induced increase in SA-β-gal positive cells and upregulation of p53, p21, and p16 expression, while ZEB1 down-regulation caused the opposite effects to be observed. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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