| Gene name: | SIRT1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HepG2 |
| Gene ID: | 23411 |
| Category: | protein coding |
| Phenotype: | Hepatocellular carcinoma |
| Experimental category: | L |
| PMID: | 23339189 |
| Experiment: | Knockdown//SA-β-gal activity assay//Colony formation assay//FACS analysis |
| Description: | The morphology of cells infected with shSIRT1 viruses was transformed into a larger, flattened shape compared to shScr and non-infected controls. Morphological changes in HepG2 cells were associated with a decrease in proliferation, measured by colony formation and alamarBlue? assays,more cells arrested in G1 and less in S phase of the cell cycle ，Their complete lack of growth was accompanied with an induction of cellular senescence as shown by enlarged cell size and expression of pH-dependent beta-galactosidase activity. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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