| Gene name: | OTX2 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | Daoy ,MED8A |
| Gene ID: | 5015 |
| Category: | protein coding |
| Phenotype: | Medulloblastoma |
| Experimental category: | HL |
| PMID: | 21047732 |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis |
| Description: | Furthermore,sustained induction of OTX2 in MED8A or DAOY cells resulted in clear morphologic changes. Cells became flattened and showed an increase in the amount of cytoplasm .β-Galactosidase activity was detected in cells withOTX2expression, but not in control cells. |
| Target gene: | IL-6//IGFBP7 |
| Official symbol(s): | IL6//IGFBP7 |
| R-AG-Target gene: | Upregulation//Upregulation |
| Subcategory: | Binding to promoter |
| Target gene experiment: | Western blot |
| Target gene description: | Indeed,the expression of senescence-associated secretory factors IL-6 (27) and IGFBP7 (28) was strongly increased after OTX2 induction . |
| Regulatory pathway: | P53 |
| R-AG-Pathway: | Activation |
| Official symbol(s): | TP53 |
| Pathway experiment: | Luciferase reporter assay |
| Pathway description: | To confirm P53 activation after OTX2 induction, we transfected wild-type and mutant reporter constructs for P53 activity into MED8A-OTX2 and MED8A-control cells. The results plotted show an up to 4-fold increase in luciferase activity after OTX2 induction in MED8A-OTX2 cells, but not in MED8A-control cells or in MED8A-OTX2 cells transfected with a mutated reporter construct. |
Annotation:
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