| Gene name: | TBX2 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | B16 |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | Activation of ER-dnTbx2 by 4-OHT induces a marked accumulation of cells staining positive for SAβ-gal activity, ~25% of clone 1 B16 HA-ER-dnTbx2-expressing cells and ~80% of the clone 2 cells. |
| Target gene: | P21 |
| Official symbol(s): | P21 |
| R-AG-Target gene: | Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | Western blot analysis using anti-p21 antibodies revealed that in cells expressing HA-ER, p21 expression was barely detectable either in the presence or absence of 4-OHT.The addition of 4-OHT to the HA-ER-dnTbx2-expressing cells led to a dramatic increase in p21 expression consistent with it acting to displace the endogenous Tbx2 from the p21 promoter.with both siRNAs efficiently down-regulating Tbx2 and consequently up-regulating p21 expression comparedwith the control siRNA . |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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