| Gene name: | PATZ1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HUVEC |
| Experiment: | SA-β-gal activity assay//Flow cytometry//BrdU assay//Knockdown |
| Description: | Knockdown of PATZ1 in young cells increased SA-β-gal staining activity.Although cell populations in S and G2/M phases were reduced, cells in G0/G1 increased in PATZ1 siRNA cells, suggesting that PATZ1 knockdown induces G0/G1 cell cycle arrest.Upregulation of PATZ1 in old cells increased cell proliferation and repressed SA-β-gal activity.PATZ1 overexpression also released old cells from G2/M cell cycle arrest, confirmed by increases in G1/G0 and S-cell populations as visualized by flow cytometry. |
| Regulatory pathway: | P53 |
| R-AG-Pathway: | -- |
| Official symbol(s): | TP53 |
| Pathway experiment: | SA-β-gal activity assay//Knockdown |
| Pathway description: | Downregulation of PATZ1 inhibited cell proliferation in p16INK4a-knockdown cells, but not in p53-knockdown cells.Transfection with PATZ1 siRNA didn't increase SA-β-gal activity in p53-knockdown cells . |
Annotation:
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