| Gene name: | TP53 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | U87,U2OS |
| Experiment: | SA-β-gal activity assay//EdU assay//Immunofluorescence |
| Description: | Nutlin-3a treatment strikingly induced cellular senescence in U87 and U2OS cells, as shown by positive senescence-associated β-galactosidase (SA-β-gal) staining, reduction of lamin B1, and reduced 5-ethynyl-2 -deoxyuridine (EdU) incorporation, p16. |
| Target gene: | EGFR//DYRK1A |
| Official symbol(s): | EGFR//DYRK1A |
| R-AG-Target gene: | Downregulation//Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//SA-β-gal activity assay |
| Target gene description: | Indeed, ectopic expression of EGFR in U87 or U2OS cells significantly attenuated senescence induced by p53 activation.Activation of p53 by Nutlin-3a led to a significant reduction in DYRK1A. |
| Regulatory pathway: | EGFR-MEK-ERK |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | EGFR-MAP2K7-MAPK1 |
| Pathway experiment: | Western blot |
| Pathway description: | Indeed, the level of phosphorylated (Activate) form of ERK was decreased in Nutlin-3a treated U87 and U2OS cells. |
Annotation:
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