| Gene name: | PTTG1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MCF-7,MCF 10A |
| Gene ID: | 9232 |
| Category: | protein coding |
| Phenotype: | Breast cancer |
| Experimental category: | HL |
| PMID: | 22789011 |
| Experiment: | SA-β-gal activity assay |
| Description: | By measuring the activity of senescence-associated b-galactosidase (SA-β-Gal), we found that hPTTG1 overexpression significantly reinforced senescence in both cell lines. |
| Target gene: | IL-8//GROA |
| Official symbol(s): | CXCL8//GROA |
| R-AG-Target gene: | Upregulation//Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Cytokine array//ELISA |
| Target gene description: | With antibody arrays, we determined that hPTTG1 overexpression led to a considerable induction in IL-8 and GROa. Because the antibody arrays were semiquantitative, we performed ELISA (enzyme-linked immunosorbent assay) analyses to confirm this result. |
| Regulatory pathway: | CXCR2-P21//NF-κB |
| R-AG-Pathway: | Upregulation//Activation |
| Official symbol(s): | CXCR2-CDKN1A//NFKB1 |
| Pathway experiment: | Dual-Luciferase reporter assay//CHIP array |
| Pathway description: | Indeed, in both cells, hPTTG1 overexpression increased the expression of the p53 and p21 . Furthermore, in hPTTG1-overexpressing MCF-7 cells, deletions of the NF-kB binding site eliminated the luciferase activity of the IL-8 and GROa promoters. These results were further confirmed by ChIP (chromatin immunoprecipitation) assay, which revealed that the binding capacities of p65 were enhanced by hPTTG1 overexpression . |
Annotation:
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