| Gene name: | IL1B |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | Osteoarthritic Chondrocyte |
| Gene ID: | 3553 |
| Category: | protein coding |
| Phenotype: | Osteoarthritis |
| Experimental category: | L |
| PMID: | 16508959 |
| Experiment: | Flow cytometry//SA-β-gal activity assay |
| Description: | In preliminary experiments (data not shown), we also confirmed the IL-1β–induced premature senescence at several concentrations of IL-1β(0.1, 1.0, 5.0, and 10.0 ng/ml) in vitro. The results indicated that premature senescence of chondrocytes was induced by IL-1β at lower concentrations as well. |
| Target gene: | CAVEOLIN-1//P38MAPK//CII |
| Official symbol(s): | CAVEOLIN-1//P38MAPK//CII |
| R-AG-Target gene: | Upregulation//Upregulation//Downregulation |
| Subcategory: | Phosphorylation |
| Target gene experiment: | qPCR//Western blot//qRT-PCR//ELISA |
| Target gene description: | Quantitative real-time RT-PCR analysis showed that both IL-1β and H2O2 enhanced caveolin 1 mRNA expression in a time-dependent manner, with the peak level occurring at 3 hours after stimulation(n ＝ 3). By Western blot analysis, we confirmed that both IL-1β and H2O2 also induced prolonged up-regulation of caveolin 1 protein expression (n = 3).Expression levels of phospho–p38 MAPK were markedly increased after IL-1β stimulation, with a peak level reached at 12 hours and sustained for more than 24 hours, whereas levels of phospho–ERK-1/2 were transiently downregulated from 6 to 12 hours.Furthermore, pretreatment with the specific p38 MAPK inhibitor SB202190 blocked the enhancement of SA–β-gal activity induced by IL-1β and H2O2.Quantitative real-time RT-PCR revealed a marked reduction in levels of expression of mRNA for CII and aggrecan in chondrocytes 3 days after stress with IL-1β or H2O2. Decreased production of CII from senescent chondrocytes after administration of either stress factor was also confirmed by ELISA (n = 4). |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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