| Gene name: | EZH2 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | SKOV3/DDP |
| Gene ID: | 2146 |
| Category: | protein coding |
| Phenotype: | Ovaria cancer |
| Experimental category: | HL |
| PMID: | 27610467 |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | The inhibition of EZH2 expression significantly downregulated H3K27me3 expression and upregulated p14, p16, p53, and pR protein expression. This suggested that EZH2 inhibition led to the activation of the INK/ARF/Rb pathway and, thereby, induced cellular senescence. Furthermore, microscopic analyses revealed the following morphological changes in the SKOV3/DDP cells in the pSUPER-EZH2 group: increased cell volume, flat granules, and β-galactosidase staining (blue), which are characteristics of cellular senescence. These changes were not observed in the blank control group. |
| Regulatory pathway: | INK-ARF-RB |
| R-AG-Pathway: | -- |
| Official symbol(s): | CDKN2A-RB1 |
| Pathway experiment: | Western blot |
| Pathway description: | The inhibition of EZH2 expression significantly downregulated H3K27me3 expression and upregulated p14, p16, p53, and pR protein expression. This suggested that EZH2 inhibition led to the activation of the INK/ARF/Rb pathway and, thereby, induced cellular senescence. |
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