| Gene name: | DIRAS3 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | ASC |
| Experiment: | Knockdown//SA-β-gal activity assay//Cell morphological analysis |
| Description: | DIRAS3 KD cells displayed a cytoplasm with thin and long processes and a significant number of these cells became flat, resembling the morphological features of the premature senescence phenotype induced by oncogenic assault in primary human fibroblasts . As demonstrated by cytochemical staining and FACS analyses using a fluorogenic substrate C12FDG,DIRAS3 KD led to a significant increase in the number of senescenceassociated β-galactosidase (SA-β-GAL) positive ASCs and to a significant increase in cell size . |
| Regulatory pathway: | AKT-MTOR |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | AKT1-MTOR |
| Pathway experiment: | Western blot//qRT-PCR//Knockdown |
| Pathway description: | To investigate the effect of DIRAS3 knock-down (KD) on Akt-mTOR signaling in ASCs we employed lentivirus mediated DIRAS3 specific shRNA . DIRAS3 KD leads to increased activity of Akt-mTOR signaling in ASCs. |
Annotation:
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