| Gene name: | ATP6V0A2 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | TIG-1 |
| Experiment: | RT-PCR//EdU assay//SA-β-gal activity assay |
| Description: | We evaluated the functional role of ATP6V A2 in cellular senescence of the TIG-1 cells. The mRNA expression of senescence markers p21 and p16, and protein expression of senescence markers p21, p16, phospho p38 and γH2AX significantly increased in ATP6V A2-silenced young and old TIG-1 cells. When DNA replication was assayed by examining 5-ethynyl-2′-deoxyuridine (EdU) incorporation in a pulse-label experiment, there was a steady decrease in EdU incorporation in ATP6V A2-silenced young TIG-1 cells and old TIG-1 cells. Growth was greatly reduced in ATP6V A2-silenced young TIG-1 cells and old TIG-1 cells. The activity of senescence-associated -β-galactosidase (SA-β-Gal) was augmented in ATP6V A2-silenced young TIG-1 cells and old TIG-1 cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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