| Gene name: | WNT7A |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | Lung |
| Cell name: | A549 |
| Gene ID: | 7476 |
| Category: | protein coding |
| Phenotype: | Lung cancer |
| Experimental category: | HL |
| PMID: | 25728679 |
| Experiment: | SA-β-gal activity assay//CCK-8 assay |
| Description: | Histological analysis of the lung sections of the wild-type and Wnt7a-null mice showed no difference in basal staining for SA-β-gal activity. However, a large number of SA-β-gal-positive cells, predominantly in the tumor area, were observed in lung sections of the wild-type mice after urethane treatment, whereas Wnt7a-null mice,on the contrary, showed reduced or no SA-β-gal staining. |
| Regulatory pathway: | SKP2-P27-PRB |
| R-AG-Pathway: | -- |
| Official symbol(s): | SKP2-PSMD9-RB1 |
| Pathway experiment: | Western Blot |
| Pathway description: | Consistent with the changesin SKP2 and p27kip1 expression, the levels of phosphorylated(p-Rb-S780) retinoblastoma proteins in Wnt7a-null mice were dramatically increased as well . Cyclin D1, which is critical for Ser780 phosphorylation of Rb ,was also upregulated in Wnt7a-null mice, along with CDK2 andCDK6, components of the CDK/cyclin complex . Similarresults were obtained when using the lung lysates of wild-typeand Wnt7a-null in FVB/NJ mice. |
Annotation:
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