| Gene name: | GADD45G |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | SK-Hep-1,SMMC-7721 |
| Gene ID: | 10912 |
| Category: | protein coding |
| Phenotype: | Hepatocellular carcinoma |
| Experimental category: | HL |
| PMID: | 26378039 |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis |
| Description: | In agreement with previous observation, we confirmed that the induction of GADD45G expression in Sk-Hep1 cells (Tet-on-GADD45G-Sk-Hep1) and SMMC-7721 cells (Tet-on-GADD45G-SMMC-7721) resulted in characteristic morphological changes common in senescent cells and a dramatic increase in the proportion of SA-β-Gal-staining positive cells. |
| Target gene: | SIP1 |
| Official symbol(s): | ZEB2 |
| R-AG-Target gene: | Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | qRT-PCR//Western blot |
| Target gene description: | Surprisingly, we found that the levels of SIP1 mRNA were significantly elevated in the cells upon GADD45G induction.The change in SIP1 mRNA expression was also reflected at the protein levels in Sk-Hep1 and SMMC-7721 cells upon GADD45G induction. In addition, GADD45G-induction of SIP1 expression similarly occurred in H-Ras V12-transformed mouse p53-/- liver progenitor cells (LPC-H-Ras V12 cells). |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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