| Gene name: | IGFBPL1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MCF-7 |
| Experiment: | Cell counting//SA-β-gal activity assay//Flow cytometry |
| Description: | Cell proliferation of MCF-7 cells was severely inhibited following IGFBP-rP1 transfection,whereas there were indistinguishable differences in cell proliferation between the control vector-transfected and the untransfected cells. On day 7, the IGFBP-rP1-transfected MCF-7 cells had an enlarged flat morphology,characteristic of senescent cells. At the same time, a significant increase in senescence-associated-β-galactosidase (SA-β-gal) activity was detected in IGFBP-rP1-transfected MCF-7 cells, as compared with control cells transfected with empty vectors or the cells that had not been transfected at all. Addition of CM from IGFBP-rP1-transfected cells to untransfected MCF-7 cells blocked cell proliferation and increased SA-β-gal activity, whereas CM from the control vector (pEGFP-N1)-transfected or untransfected cells failed to inhibit cellular proliferation and induce senescence.A significant increase in the cell population at the G /G1phase of the cell cycle was detected in IGFBP-rP1-transfected MCF-7 cells, which is one of the typical phenotypes in cellular senescence. |
| Target gene: | P21 |
| Official symbol(s): | P21 |
| R-AG-Target gene: | Upregulation |
| Subcategory: | Phosphorylation |
| Target gene experiment: | Knockdown//Western blot//Cell counting//SA-β-gal activity assay |
| Target gene description: | Basal and IGFBP-rP1-inducible p21 levels deceased dramatically in p21 knockdown MCF-7 cells.Accordingly, IGFBP-rP1-mediated pRb dephosphorylation was substantially reduced in these cells. We also examined cell proliferation and cellular senescence in response to IGFBP-rP1 in parental and p21 knockdown MCF-7 cells. The results showed that cell proliferation block and increased SA-β-gal activity in response to IGFBP-rP1 were partially reversed by p21 knockdown. These results suggest that cellular senescence induced by IGFBP-rP1 is mediated at least in part by p21 up-regulation. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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