| Gene name: | MIR584 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MGC803,SGC-7901 |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | The miR-584- 5p-mimic-transfected cells showed strong blue SA-β-gal staining, while only scattered SA-β-gal-positive cells were detected in miR-584-5p-inhibitor-transfected group compared with the corresponding control cells.The expression of p53, p21 and p16 was enhanced after miR584-5p-mimic-transfection. Conversely, the expression of p53, p21 and p16 was reduced after miR-584-5p-inhibitor transfection. |
| Target gene: | WWP1//P53 |
| Official symbol(s): | WWP1//P53 |
| R-AG-Target gene: | Downregulation//Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | SA-β-gal activity assay//Western blot//Flow cytometry |
| Target gene description: | Intriguingly, co-transfection of MGC803 cells with miR-584-mimic and either LV-WWP1 or LV-NC showed that ectopic expression of WWP1 effectively reversed the promotion of cellular senescence resulting from miR-584-5p overexpression. Similarly, the effects of miR584-5p knockdown in SGC7901 were counteracted by WWP1 downregulation. The induction of SA-β-Gal positively stained cells by miR-584-5p was also reduced by treatment of pifithrin-α, indicating the involvement of p53 in miR-584-5p-induced cellular senescence.In accordance with the analysis of GC tissues, Western blot analysis showed an inverse correlation between WWP1 expression and miR-584-5p expression in GC cell lines and GES-1 cells.The expression of p53, p21 and p16 was enhanced after miR-584-5p-mimic-transfection. |
| Regulatory pathway: | TGFβ |
| R-AG-Pathway: | Activation |
| Official symbol(s): | TGFB1 |
| Pathway experiment: | Western blot//Immunofluorescence |
| Pathway description: | Western blot analysis of the expression of WWP1 and key proteins involved in TGFβ signaling pathway showed that the protein levels of TβR1, Smad2, Smad4, and the CDK inhibitor p15 were increased after miR-584-5p-mimic transfection, while the opposite effects were observed after miR-584-5p-inhibitor transfection.However, the expression of TβR1, Smad2, Smad4, and p15 was decreased in LY364947 treated cells compared with that of the control group. Furthermore, immunofluorescence assays revealed a dramatically positive correlation between the levels of miR-584-5p and TGFβ. |
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