Sample overview

Sample ID: Sample_1384
Biosample name: iPSC
Biosample type: Induced pluripotent stem cell line
Tissue type: Other
Cancer type: Other
Region number: 123725
Length: 61442.215 kb
GEO/SRA ID: SRR8393559
Mean insert size: 181.423
Standard deviation: 125.495
TSS enrichment score: 67.59250
FRiP: 0.57874
Diasgnostic plot
The percentages of read pairs in FR, RF, and TANDEM orientations.


TF footprint of iPSC


Peak annotation visualization of iPSC

Plot A

Plot B

Plot A: a pie chart of annotated genomic features.
Note A: the proportion of annotating genomic region (Promoter, 5'UTR, 3'UTR, Exon, Intron, Downstream and Intergenic).
Plot B: a heatmap of peaks binding to TSS region.
Note B: the profile of ATAC peaks binding to TSS region (+/- 1kb).

Software and parameters used

Read mapping: bowtie2(v2.2.5) -p 10 -X 2000 -x -S
Duplicates Removing: picard MarkDuplicates REMOVE_DUPLICATES=true ASSUME_SORTED=true
Peak calling: macs2(2.1.2) --broad--SPMR --nomodel --extsize 200 -q 0.01
Trimming the adapter: trim_galore --phred33 --stringency 3 --length 20